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KMID : 0364219900330030303
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Korean Journal of Zoology
1990 Volume.33 No. 3 p.303 ~ p.309
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Overexpression and Characterization of Eukaryotic Peptide Hormone Precursors in E. Coli.
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Hong Seung-Hwan
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Abstract
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In order to have a handle on the availability of eukarvotic peptide hormone precursors, a cDNA encoding angler fish prepro-SRIF I was manipulated so that it can be produced in large quantity from heterologous E. coli cells. Using T7 overexpression system, fusion constructs between the T7 phage coat protein Sl0 and the prepro-SRIF were made and modified as desired. From the host E. coli strain, BL21 DE3, harboring these plasmid constructs, three different SRIF related polypeptides were expressed in large amount and characterized. The results confirm the exact construction and authenticity of the overexpressed proteins from E. coli cells. The importance of this heterologous overexpression in hard to get peptide hormone precursors as well as the suitability of the target peptide hormone SRIF for this approach are discussed.
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KEYWORD
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SRIF , Heterologous overexpression , Eukaryotic peptide precursor
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